Taurohenodezoksiholat 6α-hidroksilaza
(преусмерено са CYP3A4)
Taurohenodezoksiholat 6α-hidroksilaza (EC 1.14.13.97, CYP3A4, CYP4A21, taurohenodezoksiholat 6alfa-monooksigenaza) je enzim sa sistematskim imenom taurohenodezoksiholat,NADPH:kiseonik oksidoreduktaza (6alfa-hidroksilacija).[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Taurohenodezoksiholat 6α-hidroksilaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 1.14.13.97 | ||||||||
| CAS broj | 105669-85-0 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- (1) taurohenodezoksiholat + NADPH + H+ + O2 taurohioholat + NADP+ + H2O
- (2) lithoholat + NADPH + H+ + O2 hiodezoksiholat + NADP+ + H2O
Ovaj enzim je hem-tiolatni protein (P-450). Za maksimalnu aktivnost je neophodan citohrom b5.
Reference
уреди- ^ Araya, Z. & Wikvall, K. (1999). „6α-Hydroxylation of taurochenodeoxycholic acid and lithocholic acid by CYP3A4 in human liver microsomes”. Biochim. Biophys. Acta. 1438: 47—54. PMID 10216279.
- ^ Araya, Z., Hellman, U. and Hansson, R. (1995). „Characterisation of taurochenodeoxycholic acid 6α-hydroxylase from pig liver microsomes”. Eur. J. Biochem. 231: 855—861. PMID 7649186.
- ^ Kramer, W., Sauber, K., Baringhaus, K.H., Kurz, M., Stengelin, S., Lange, G., Corsiero, D., Girbig, F., Konig, W. and Weyland, C. (2001). „Identification of the bile acid-binding site of the ileal lipid-binding protein by photoaffinity labeling, matrix-assisted laser desorption ionization-mass spectrometry, and NMR structure”. J. Biol. Chem. 276: 7291—7301. PMID 11069906.
- ^ Lundell, K., Hansson, R. and Wikvall, K. (2001). „Cloning and expression of a pig liver taurochenodeoxycholic acid 6α-hydroxylase (CYP4A21): a novel member of the CYP4A subfamily”. J. Biol. Chem. 276: 9606—9612. PMID 11113117.
- ^ Lundell, K. & Wikvall, K. (2003). „Gene structure of pig sterol 12α-hydroxylase (CYP8B1) and expression in fetal liver: comparison with expression of taurochenodeoxycholic acid 6α-hydroxylase (CYP4A21)”. Biochim. Biophys. Acta. 1634: 86—96. PMID 14643796.
- ^ Russell, D.W. (2003). „The enzymes, regulation, and genetics of bile acid synthesis”. Annu. Rev. Biochem. 72: 137—174. PMID 12543708.
Literatura
уреди- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
Spoljašnje veze
уреди- Taurochenodeoxycholate+6alpha-hydroxylase на US National Library of Medicine Medical Subject Headings (MeSH)
