Сортаза-А
Sortaza-A (EC 3.4.22.70, SrtA, SrtA protein, SrtA sortaza) je enzim.[1][2][3] Ovaj enzim katalizuje sledeću hemijsku reakciju
| Sortaza-A | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.4.22.70 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- Ovaj enzim enzim katalizuje reakciju sortiranja ćelijskog zida u kojoj se površinski protein sa signalom sortiranja koji sadrži LPXTG motif razlaže između Thr i Gly ostataka. Rezultujući treoninski karboksilni kraj proteina je kovalentno vezan za pentaglicin peptidoglikana.
Ovaj enzim pripada peptidaznoj familiji C60.
Reference
уреди- ^ Ton-That, H., Liu, G., Mazmanian, S.K., Faull, K.F. and Schneewind, O. (1999). „Purification and characterization of sortase, the transpeptidase that cleaves surface proteins of Staphylococcus aureus at the LPXTG motif”. Proc. Natl. Acad. Sci. USA. 96: 12424—12429. PMID 10535938.
- ^ Zong, Y., Bice, T.W., Ton-That, H., Schneewind, O. and Narayana, S.V. (2004). „Crystal structures of Staphylococcus aureus sortase A and its substrate complex”. J. Biol. Chem. 279: 31383—31389. PMID 15117963.
- ^ Race, P.R., Bentley, M.L., Melvin, J.A., Crow, A., Hughes, R.K., Smith, W.D., Sessions, R.B., Kehoe, M.A., McCafferty, D.G. and Banfield, M.J. (2009). „Crystal structure of Streptococcus pyogenes sortase A: implications for sortase mechanism”. J. Biol. Chem. 284: 6924—6933. PMID 19129180.
Literatura
уреди- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
- William P. Jencks (1987). Catalysis in Chemistry and Enzymology. Dover Publications. ISBN 0486654605.
