D-Ala-D-Ala dipeptidaza
D-Ala-D-Ala dipeptidaza (EC 3.4.13.22, D-alanil-D-alanin dipeptidaza, vanX D-Ala-D-Ala dipeptidaza, VanX) je enzim.[1][2][3][4][5][6] Ovaj enzim katalizuje sledeću hemijsku reakciju
| D-Ala-D-Ala dipeptidaza | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifikatori | |||||||||
| EC broj | 3.4.13.22 | ||||||||
| Baze podataka | |||||||||
| IntEnz | IntEnz pregled | ||||||||
| BRENDA | BRENDA pristup | ||||||||
| ExPASy | NiceZyme pregled | ||||||||
| KEGG | KEGG pristup | ||||||||
| MetaCyc | metabolički put | ||||||||
| PRIAM | profil | ||||||||
| Strukture PBP | RCSB PDB PDBe PDBj PDBsum | ||||||||
| |||||||||
- D-Ala-D-Ala + H2O 2 D-Ala
Ovaj enzim je zavistan od Zn2+ jona.
Reference
уреди- ^ Reynolds, P.E., Depardieu, F., Dutka-Malen, S., Arthur, M. and Courvalin, P. (1994). „Glycopeptide resistance mediated by enterococcal transposon Tn1546 requires production of VanX for hydrolysis of D-alanyl-D-alanine”. Mol. Microbiol. 13: 1065—1070. PMID 7854121.
- ^ Wu, Z., Wright, G.D. and Walsh, C.T. (1995). „Overexpression, purification, and characterization of VanX, a D-, D-dipeptidase which is essential for vancomycin resistance in Enterococcus faecium BM4147”. Biochemistry. 34: 2455—2463. PMID 7873524.
- ^ McCafferty, D.G., Lessard, I.A. and Walsh, C.T. (1997). „Mutational analysis of potential zinc-binding residues in the active site of the enterococcal D-Ala-D-Ala dipeptidase VanX”. Biochemistry. 36: 10498—10505. PMID 9265630.
- ^ Bussiere, D.E., Pratt, S.D., Katz, L., Severin, J.M., Holzman, T. and Park, C.H. (1998). „The structure of VanX reveals a novel amino-dipeptidase involved in mediating transposon-based vancomycin resistance”. Mol. Cell. 2: 75—84. PMID 9702193.
- ^ Tan, A.L., Loke, P. and Sim, T.S. (2002). „Molecular cloning and functional characterisation of VanX, a D-alanyl-D-alanine dipeptidase from Streptomyces coelicolor A3(2)”. Res. Microbiol. 153: 27—32. PMID 11881895.
- ^ Matthews, M.L., Periyannan, G., Hajdin, C., Sidgel, T.K., Bennett, B. and Crowder, M.W. (2006). „Probing the reaction mechanism of the D-ala-D-ala dipeptidase, VanX, by using stopped-flow kinetic and rapid-freeze quench EPR studies on the Co(II)-substituted enzyme”. J. Am. Chem. Soc. 128: 13050—13051. PMID 17017774.
Literatura
уреди- Nicholas C. Price; Lewis Stevens (1999). Fundamentals of Enzymology: The Cell and Molecular Biology of Catalytic Proteins (Third изд.). USA: Oxford University Press. ISBN 019850229X.
- Eric J. Toone (2006). Advances in Enzymology and Related Areas of Molecular Biology, Protein Evolution (Volume 75 изд.). Wiley-Interscience. ISBN 0471205036.
- Branden C; Tooze J. Introduction to Protein Structure. New York, NY: Garland Publishing. ISBN 0-8153-2305-0.
- Irwin H. Segel. Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems (Book 44 изд.). Wiley Classics Library. ISBN 0471303097.
Spoljašnje veze
уреди- D-Ala-D-Ala+dipeptidase на US National Library of Medicine Medical Subject Headings (MeSH)
